
DFG Collaborative Research Centre 1444
This Collaborative Research Centre aims to unravel the basic mechanisms that differentiate between success and failure in regeneration of musculoskeletal tissue using bone healing as a role model.
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Subproject 3 - Principal Investgators

CRC 1444 Representative of partner university & Education of early career scientists, Subprojects 3 & 4 | RU 2165 Subprojects 3

ECM coding and reading: alterations of ECM properties under compromised conditions and consequences for regeneration

In order to understand alterations of extracellular matrix properties under compromised conditions and consequences for regeneration, Petersen and Knaus will investigate extracellular matrix coding and reading in their project. They will elucidate how information encoded in the extracellular matrix influences cell function in the context of bone regeneration. Specifically, they aim at deciphering alterations of the here-defined "matrix code" under compromised conditions related to aging (altered immune status, bone marrow adiposity) in vitro. They hypothesise that precursor cells, if recruited from a compromised bone marrow niche to a bone defect would deposit a compromised extracellular matrix that is modulated additionally by the immune system. The resulting compromised extracellular matrix in turn alters progenitor cell function and thereby entails an impaired healing pathway.

Growth factor - receptor affinities
Inflammatory Response | Force Transmission & Sensing
Receptor complexes of the TGFβ/BMP superfamily can be visualised by N-terminal halo and SNAP labelled receptors in combination with fluorescently labelled growth factors.
Jatzlau et al. Commun Biol 2023 | In collaboration with subproject P04

Angiogenesis
Inflammatory Response | Force Transmission & Sensing
2D and 3D Traction Force Microscopy (TFM) were developed to study mechanical forces during angiogenesis of iPS-derived endothelial cells.
In collaboration with subproject P10
Team
Correlative approach to study endothelial junction architecture and sprouting angiogenesis in iPSC-derived endothelial cells for different disease models

Resolving the Activin A receptor complex and its endothelial function in Fibrodysplasia Ossificans Progressiva (FOP)

Extracellular matrix coding and reading: Alterations under compromised conditions and consequences for cell behavior in tissue regeneration

Deciphering the brain integrity and modulation of endothelial cell junctions under the control of BMP

Shear-stress dependent modulation of BMP-induced transcription

Publications
- Authors:Jatzlau, J.; Burdzinski, W.; Trumpp, M.; Obendorf, L.; Rossmann, K.; Ravn, K.; Hyvonen, M.; Bottanelli, F.; Broichhagen, J.; Knaus, P.
Journal:Commun Biol Year:2023; Volume:6Issue:(1):Pages:34.
Title:A versatile Halo- and SNAP-tagged BMP/TGFbeta receptor library for quantification of cell surface ligand binding