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DFG Collaborative Research Centre 1444

This Collaborative Research Centre aims to unravel the basic mechanisms that differentiate between success and failure in regeneration of musculoskeletal tissue using bone healing as a role model.

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Subproject 14 - Principal Investgators

Prof. Dr. Anja Hauser

CRC 1444 Subproject 14 | RU 2165 Subproject 7

Prof. Dr. Raluca Niesner

CRC 1444 Subproject 14 | RU 2165 Subproject 7

Interactions between myeloid and stromal cells during bone healing under compromising conditions

The interactions between myeloid and stromal cells during bone healing under compromising conditions will be visualised by Hauser and Niesner. Myeloid cells can integrate various types of signals such as interactions with hematopoietic and stromal cells, as well as metabolic conditions, and mechanical stimuli in order to differentiate during bone regeneration. Combining the development of state-of-the-art technology for functional, longitudinal intravital imaging with expertise in immunology and bone biology while using genetic and pharmacological tools will allow to dissect the signals acting on myeloid cell differentiation at the site of injury in fracture models. Analysing the spatio-temporal heterogeneity of myeloid cells and their microenvironment during the course of bone healing, followed by intravital investigation of the correlation between myeloid cell functions and cellular metabolism and an investigation of the molecular mechanisms underlying mechanosensing in myeloid cells will be used to analyse the impact of the microenvironment on myeloid cell phenotype and functions during the phases of bone healing via cellular interactions, local metabolic conditions and mechanical cues.

Most important results

Three photon microscopy

Inflammatory Response | Energy Supply & Consumption

Three-photon microscopy allows optical analysis of cellular and extracellular changes in bone and bone marrow in intact long tubular bones.

Metabolic heterogeneity

Inflammatory Response | Energy Supply & Consumption

NADH/NADPH fluorescence lifetime LIMB reveals metabolic heterogeneity in myeloid cell populations during bone healing.

Close interaction

Inflammatory Response | Energy Supply & Consumption

3D analyses of the whole, cleared bone show a close interaction of myeloid cells and the vascular system.


Alexander Fiedler (PhD Student)

Development of in vivo fluorescence lifetime imaging techniques for analyzing the immunometabolism during bone healing

Alina Liebheit (Doctoral Researcher)

Analyse des Stromakompartiments im Knochenmark bei der Knochenheilung

Till Mertens (Medical Doctoral Researcher)

3D analysis of vascularization and the interaction between macrophages and endothelial cells during fracture healing

Dr. Asylkhan Rakhymzhan (Postdoc)

Development of an intravital three-photon microscopy method in long mouse bones to study the effect of mechanical stress down to the cellular level

Dr. Jay Roodselaar (Postdoc, until 05/2023)





Founded by the DFG (Project Number: 427826188)
Funding Period 2021-2024