DFG Collaborative Research Centre 1444
This Collaborative Research Centre aims to unravel the basic mechanisms that differentiate between success and failure in regeneration of musculoskeletal tissue using bone healing as a role model.
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Interactions between myeloid and stromal cells during bone healing under compromising conditions
The interactions between myeloid and stromal cells during bone healing under compromising conditions will be visualised by Hauser and Niesner. Myeloid cells can integrate various types of signals such as interactions with hematopoietic and stromal cells, as well as metabolic conditions, and mechanical stimuli in order to differentiate during bone regeneration. Combining the development of state-of-the-art technology for functional, longitudinal intravital imaging with expertise in immunology and bone biology while using genetic and pharmacological tools will allow to dissect the signals acting on myeloid cell differentiation at the site of injury in fracture models. Analysing the spatio-temporal heterogeneity of myeloid cells and their microenvironment during the course of bone healing, followed by intravital investigation of the correlation between myeloid cell functions and cellular metabolism and an investigation of the molecular mechanisms underlying mechanosensing in myeloid cells will be used to analyse the impact of the microenvironment on myeloid cell phenotype and functions during the phases of bone healing via cellular interactions, local metabolic conditions and mechanical cues.
Development of in vivo fluorescence lifetime imaging techniques for analyzing the immunometabolism during bone healing
Analyse des Stromakompartiments im Knochenmark bei der Knochenheilung
3D analysis of vascularization and the interaction between macrophages and endothelial cells during fracture healing
Development of an intravital three-photon microscopy method in long mouse bones to study the effect of mechanical stress down to the cellular level